Influence Of Endogenous Biotin On Immunohistochemical Staining
The emergence and development of immunohistochemistry brought a revolution to traditional pathology
. It made pure pathology developed into modern pathology in a combination of stapes and immune signaling. In the process of the development and application of immunohistochemical techniques, the introduction of heating antigen retrieval techniques makes the closed antigen re-exposure in formalin-fixed and paraffin-embedded. So that many monoclonal antibodies can be used to detect expression of paraffin buried organization. Therefore, it greatly improves and promotes the use and development of immunohistochemistry.However, while the benefits of heating antigen retrieval technique for immunohistochemistry, it also brings side effects. And, this side effect has not yet been fully aware of. When using biotin - avidin or biotin - streptavidin avidin detection system prone to false positives. Comparatively speaking, the people of endogenous peroxidase has been fully understood, and effective over hydrogen peroxide to eliminate peroxidase activity in the organization, so as to achieve the purpose of avoiding non-specific staining, however, little is known about the impact of endogenous biotin, almost did not take any preventive measures. In fact, the endogenous biological factors impact and the extent of immunohistochemical staining is much higher than the endogenous peroxidase.
The results show that cosmetics material biotin with repairing and exposure can be fully closed by 20-30% of eggs white. The principle is that the egg contains avidin, avidin and bio-known as a very strong affinity, and a combination of both, closed the endogenous biotin all binding sites, to avoid endogenous biotin and ABC. LSAB or SP detection system, avidin or streptavidin avidin's binding, and thus eliminate the non-specific effects of endogenous biotin. As early as 1916, scientists discovered that a toxic substance in the egg protein, until 1927 found that a certain food protein can be prevented toxicity. After the study, about to such anti-protein toxic substances - biotin separation. The structure of the biotin to be revealed in 1936, and 1943 in the laboratory synthesis of such substances. Biotin is a ring chemical like urea, the structure of a thiophenol ring, such chemical substances eight-shaped isomers, but only right-handed biotin exists in nature, and has the function of vitamins.
Biotin is a colorless, needle-like substance, slightly soluble in cold water, more easily dissolved in alcohol, but insoluble in organic solvents. Biotin was stable to heat, is not damaged by acid or alkaline. Biotin wide range of trace amounts of content in the tissues of animals and plants, especially abundant in yeast, liver, kidney. The human requirement for biotin difficult to quantitative research found that food in excess of biotin from the urine, while the bacteria in the gut will also create a biotin. Biotin absorbed in the small intestine parts of the well, each cell can be said to contain biotin, in particular, more content in the liver and kidney tissue.
Biotin functions include cell growth; the growth of fatty acids, metabolisms of protein, fat, and carbohydrate and the use of vitamin B group. Synergistic substances contain vitamin B complex, vitamin B12, folic acid, pantothenic acid, vitamin C and sulfur. The antagonism substances are alcohol, coffee and raw protein. The biotin supplier finds that there are rich biotin contents in cells which have vigorous metabolism and rich mitochondria. So in epithelium-derived cells and tumor cells, they are prone to have positive reactions of biotin. These false positive reactions can easily be regarded as specific reaction, thus affecting judgment of the results. Therefore, if there are positive brown granules in the cytoplasm in practical works, people should take into account the possibility of endogenous biotin. It is better to do further confirmations if the conditions permits.Source:http://www.cosprm.com
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